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DNA cell cycle studies in uveal melanoma
1Department of Ophthalmology, Francis I. Proctor Foundation, University of California, San Francisco 94143.
American Journal of Ophthalmology
|January 15, 1989
Summary
Radiation therapy significantly reduces the reproductive integrity of uveal melanomas. Bromodeoxyuridine uptake and fine needle aspiration biopsy can help distinguish successfully treated tumors from those with radiation-induced changes.
Area of Science:
- Ophthalmology
- Oncology
- Radiation Oncology
Background:
- Uveal melanoma is the most common primary intraocular malignancy.
- Treatment options include surgery and various forms of radiation therapy.
- Assessing treatment effectiveness and differentiating tumor regrowth from treatment side effects can be challenging.
Purpose of the Study:
- To evaluate the impact of different radiation doses on the reproductive integrity of uveal melanomas.
- To assess the utility of bromodeoxyuridine uptake and fine needle aspiration biopsy in evaluating treatment response.
Main Methods:
- Histological study of uveal melanomas treated with surgery alone, low-dose preenucleation radiation, or high-dose charged-particle beam therapy.
- Measurement of bromodeoxyuridine uptake in tumor cells.
- Assessment of tumor growth in tissue culture.
- Evaluation of fine needle aspiration biopsy techniques.
Main Results:
- Irradiated melanomas showed significantly lower bromodeoxyuridine uptake compared to non-irradiated tumors (P < .0001).
- Tissue culture growth of irradiated tumors was significantly reduced (P < .007).
- These findings indicate destruction of reproductive integrity in helium ion-irradiated melanomas.
Conclusions:
- High-dose charged-particle radiation therapy effectively destroys the reproductive capacity of uveal melanomas.
- Bromodeoxyuridine uptake and fine needle aspiration biopsy are potentially valuable tools for differentiating successful radiation treatment from radiation-induced vasculopathy.