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Thermostable exoshells fold and stabilize recombinant proteins.

Siddharth Deshpande1,2,3, Nihar D Masurkar1,2, Vallerinteavide Mavelli Girish1,2

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Engineered a thermostable protein nanoparticle (tES) to enhance recombinant protein expression and stability. This technology significantly improves protein folding yields and protects enzymes from denaturation, enabling controlled release.

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Area of Science:

  • Biotechnology
  • Protein Engineering
  • Nanotechnology

Background:

  • Recombinant protein expression and stabilization are crucial for biological research and applications.
  • Existing methods often face challenges in achieving high yields and maintaining protein integrity.
  • Novel approaches are needed to improve the efficiency and robustness of protein-based technologies.

Purpose of the Study:

  • To engineer a novel thermostable protein nanoparticle (tES) system.
  • To enhance the expression and stabilization of various recombinant proteins.
  • To evaluate the functional recovery and protective capabilities of the tES system.

Main Methods:

  • Engineering of a thermostable protein nanoparticle (tES).
  • Encapsulation of model proteins: green fluorescent protein (GFPuv), horseradish peroxidase (HRPc), and Renilla luciferase (rLuc).
  • Assessment of in vitro folding yields, enzymatic activity, spectral properties, and resistance to denaturation.

Main Results:

  • The tES system improved functional in vitro folding yields of encapsulated proteins by approximately 100-fold.
  • Encapsulated enzymes retained metabolic activity, with evidence of 4.5-nm pores in the tES shell.
  • Encapsulated proteins showed enhanced resistance to thermal, organic, chaotropic, and proteolytic denaturation.
  • Thermolabile proteins could be released from the tES assembly using mild pH titration and proteolysis.

Conclusions:

  • The engineered thermostable protein nanoparticle (tES) is an effective platform for improving recombinant protein expression and stability.
  • tES provides significant protection against various denaturing conditions while preserving protein function.
  • The tES system offers a versatile tool for protein stabilization, controlled delivery, and enhanced biotechnological applications.