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Updated: Feb 18, 2026

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Published on: November 5, 2014
A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete
Sarah H Hewitt1, Jonathan Parris, Romain Mailhot
1Department of Chemistry, Loughborough University, Epinal Way, Loughborough, LE11 3TU, UK. s.j.butler@lboro.ac.uk.
Abstract:
We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.
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