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Updated: Feb 18, 2026
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
Ultrasonic-based membrane aided sample preparation of urine proteomes
Jemmyson Romário Jesus1, Hugo M Santos2, H López-Fernández3
1Spectrometry, Sample Preparation and Mechanization Group - GEPAM, Institute of Chemistry, University of Campinas - UNICAMP, PO Box 6154, CEP 13083-970 Campinas, Brazil; National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas - UNICAMP, PO Box 6154, CEP 13083-970 Campinas, Brazil; UCIBIO-REQUIMTE, Chemistry Department, Faculty of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.
A novel ultrafast ultrasonic method rapidly analyzes urine proteins for label-free quantification. This technique can differentiate between male and female urine samples based on protein content in just three hours.
Area of Science:
- Proteomics
- Analytical Chemistry
- Biotechnology
Background:
- Urine proteomics offers insights into physiological states.
- Traditional protein analysis methods are time-consuming and complex.
- Need for rapid, efficient protein quantification in biological samples.
Purpose of the Study:
- Develop an ultrafast, label-free method for shotgun proteomics and protein quantification in urine.
- Accelerate the protein digestion process for faster sample analysis.
- Enable rapid differentiation of urine samples based on gender-specific protein profiles.
Main Methods:
- Proteins separated using nitrocellulose membranes.
- In-membrane protein digestion accelerated by a sonoreactor ultrasonic device (4 minutes).
- Shotgun proteomics and label-free quantification performed within a 3-hour pipeline.
Main Results:
- Successfully identified 232 proteins in male and 226 in female urine samples.
- Distinguished between male and female urine based on protein content.
- Identified 70 male-unique and 64 female-unique proteins, with 13 common proteins showing statistically significant differences (p < 0.05).
Conclusions:
- The developed ultrasonic-based method provides a fast, efficient, and straightforward approach for urine proteomics.
- This technique aligns with analytical minimalism, reducing time, cost, and waste.
- Potential for rapid biomarker discovery and gender-based differentiation using urine proteomic profiles.
