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Updated: Feb 18, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Long-wavelength fluorescent boronate probes for the detection and intracellular imaging of peroxynitrite
Adam C Sedgwick1, Hai-Hao Han, Jordan E Gardiner
1Department of Chemistry, University of Bath, Bath, BA2 7AY, UK. t.d.james@bath.ac.uk s.d.bull@bath.ac.uk.
Abstract:
Two boronate fluorescent probes have been developed for the detection of peroxynitrite (TCFB1 and TCFB2). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459).

