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Updated: Jul 22, 2026

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
Identification of cholera toxin-binding sites in the nucleus of intestinal epithelial cells
M E Parkinson1, C G Smith, P B Garland
1Department of Biochemistry, University of Edinburgh, Scotland.
Abstract:
Post-embedding immunogold electron microscopy shows several binding sites for cholera toxin in mouse intestinal epithelial cells, particularly in the heterochromatin of the nucleus as well as in the plasma membrane. Anti-ganglioside GM1 antibodies also bound to the nucleus, but did not interfere with the binding of toxin. 125I-labelled toxin bound specifically to a nuclear preparation from rabbit intestinal cells.
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