Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Permeable Liquid Metal-Based Fibrous Patch with Dual-Bond Interconnects for Reliable Sensing and Closed-Loop Wound Care.

ACS nano·2026

Same author

Linking Electron Cloud Potential Wells to Achieve Ultrahigh Output Current in a Triboelectric Nanogenerator.

Advanced materials (Deerfield Beach, Fla.)·2025

Same author

Autonomous, Moisture-Driven Flexible Electrogenerative Dressing for Enhanced Wound Healing.

Advanced materials (Deerfield Beach, Fla.)·2025

Same author

Charge Dispersion Strategy for High-Performance and Rain-Proof Triboelectric Nanogenerator.

Advanced materials (Deerfield Beach, Fla.)·2023

Same author

MnO<sub>4</sub><sup>-</sup>-Triggered Immediate-Stable Real-Time Fluorescence Immunosensor with High Response Speed and Low Steady-State Error.

Analytical chemistry·2023

Same author

Green Flexible Electronics: Natural Materials, Fabrication, and Applications.

Advanced materials (Deerfield Beach, Fla.)·2023

Same journal

Recent developments of textile-based triboelectric nanogenerators for smart sports applications.

Biosensors & bioelectronics·2026

Same journal

One-Tube RPA-CRISPR-Cas13a assay with rational design for single-molecule detection of waterborne viruses in drinking water treatment.

Biosensors & bioelectronics·2026

Same journal

AI-driven photophysics-aware design of fluorescent probes with applications in α-synuclein biosensing and inhibitor screening.

Biosensors & bioelectronics·2026

Same journal

Three-dimensional helical integration of high-density linear microelectrode arrays and their cross-tissue applications.

Biosensors & bioelectronics·2026

Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026

Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 18, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Paper-based fluorogenic devices for in vitro diagnostics.

Meirong Wu¹, Qiongyu Lai¹, Qiang Ju¹

¹Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (Nanjing Tech), 30 South Puzhu Road, Nanjing 211816, PR China.

Biosensors & Bioelectronics

|November 21, 2017

Summary

This summary is machine-generated.

Paper-based fluorogenic devices offer a cost-effective, sensitive, and user-friendly approach for in vitro diagnostics (IVD). These equipment-free devices show great potential for improving global healthcare, especially in resource-limited settings.

Keywords:

Bioassays Fluorogenic detection In vitro diagnostics Paper-based devices Point of care

More Related Videos

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Related Experiment Videos

Last Updated: Feb 18, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Area of Science:

Analytical Chemistry
Biomedical Engineering
Materials Science

Background:

In vitro diagnostics (IVD) are critical for disease detection but face challenges in affordability and sensitivity.
Paper-based analytical devices (PADs) offer a promising low-cost alternative for diagnostic applications.
Fluorescent detection methods on PADs are gaining attention for their high sensitivity and selectivity.

Purpose of the Study:

To provide an overview of paper-based fluorogenic devices as an emerging IVD technology.
To summarize and compare various fluorescent materials utilized in these devices.
To review the diverse applications of paper-based fluorogenic devices in diagnostics.

Main Methods:

Review of literature on paper-based fluorogenic devices for IVD.
Comparison of different fluorescent materials including dyes, quantum dots, metal nanoclusters, upconversion nanoparticles, and carbon dots.
Analysis of various IVD applications such as detection of nucleic acids, proteins, and cells.

Main Results:

Paper-based fluorogenic devices are inexpensive, sensitive, selective, user-friendly, and equipment-free.
Fluorescent detection enhances sensitivity and selectivity in paper-based diagnostics.
A wide range of fluorescent materials can be integrated into these devices for diverse applications.

Conclusions:

Paper-based fluorogenic devices represent a cost-effective and high-performance analytical method for IVD.
These devices have the potential to significantly improve healthcare in developing countries and emergency situations.
The versatility of fluorescent materials and applications makes paper-based fluorogenic devices a promising platform for point-of-care diagnostics.