Oxytocin-receptor-expressing neurons in the parabrachial nucleus regulate fluid intake

Brain regions that regulate fluid satiation are not well characterized, yet are essential for understanding fluid homeostasis. We found that oxytocin-receptor-expressing neurons in the parabrachial nucleus of mice (Oxtr^PBN neurons) are key regulators of fluid satiation. Chemogenetic activation of Oxtr^PBN neurons robustly suppressed noncaloric fluid intake, but did not decrease food intake after fasting or salt intake following salt depletion; inactivation increased saline intake after dehydration and hypertonic saline injection. Under physiological conditions, Oxtr^PBN neurons were activated by fluid satiation and hypertonic saline injection. Oxtr^PBN neurons were directly innervated by oxytocin neurons in the paraventricular hypothalamus (Oxt^PVH neurons), which mildly attenuated fluid intake. Activation of neurons in the nucleus of the solitary tract substantially suppressed fluid intake and activated Oxtr^PBN neurons. Our results suggest that Oxtr^PBN neurons act as a key node in the fluid satiation neurocircuitry, which acts to decrease water and/or saline intake to prevent or attenuate hypervolemia and hypernatremia.