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Real time complete Stokes polarimetric imager based on a linear polarizer array camera for tissue polarimetric
Ji Qi1,2, Chao He1,2, Daniel S Elson1,2
1Hamlyn Centre for Robotic Surgery, Institute of Global Health Innovation, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
Biomedical Optics Express
|December 1, 2017
Summary
A new compact Stokes polarimetric imager was developed for real-time tissue imaging. This tool revealed that collagen fiber polarization properties change during dehydration, requiring careful sample preparation for accurate polarimetric microscopy.
Area of Science:
- Biomedical Optics
- Imaging Science
- Materials Science
Background:
- Tissue polarimetric imaging, using Mueller matrices or Stokes vectors, is valuable for surgical and diagnostic applications.
- Existing methods often lack real-time capabilities or compactness for versatile use.
Purpose of the Study:
- To introduce a compact, multi-wavelength, frame-rate capable Stokes polarimetric imager.
- To validate the imager's performance and demonstrate its application in studying tissue dehydration.
Main Methods:
- Development of a compact complete Stokes polarimetric imager with multi-wavelength capabilities.
- Validation using standard polarizing components.
- Application as a polarization state analyzer and standalone Stokes imager to observe bovine tendon dehydration.
Main Results:
- The developed imager demonstrated suitability for real-time applications.
- Observed changes in polarization properties of collagen-rich tissues correlated with the degree of dehydration.
- Bovine tendon tissue dehydration significantly altered its polarization characteristics.
Conclusions:
- The compact Stokes polarimetric imager is effective for real-time tissue analysis.
- Tissue dehydration critically affects polarization properties, particularly in collagen-rich samples.
- Controlled dehydration is essential for reliable polarimetric imaging and microscopy of biological tissues.
