Related Experiment Video
Updated: Feb 17, 2026
Isolation of Murine Lymph Node Stromal Cells
Published on: August 19, 2014
Harnessing the lymph node microenvironment
Natalie A O'Neill1, Haleigh B Eppler2, Christopher M Jewell2
1Department of Surgery, University of Maryland School of Medicine, Baltimore.
Purpose Of Review:
To evaluate role of the lymph node in immune regulation and tolerance in transplantation and recent advances in the delivery of antigen and immune modulatory signals to the lymph node.
Recent Findings:
Lymph nodes are a primary site of immune cell priming, activation, and modulation, and changes within the lymph node microenvironment have the potential to induce specific regulation, suppression, and potentially tolerance. Antigen enters the lymph node either from tissues via lymphatics, from blood via high endothelial venules, or directly via injection. Here we review different techniques and materials to deliver antigen to the lymph node including microparticles or nanoparticles, ex-vivo antigen presenting cell manipulation, and use of receptor conjugation for specific intralymph node targeting locations.
Summary:
The promising results point to powerful techniques to harness the lymph node microenvironment and direct systemic immune regulation. The materials, techniques, and approaches suggest that translational and clinical trials in nonhuman primate and patients may soon be possible.
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