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Hypertonic Saline Injection Model of Experimental Glaucoma in Rats
John C Morrison1, Elaine C Johnson2, William O Cepurna2
1Casey Eye Institute, Oregon Health and Science University, 3375 S.W. Terwilliger Blvd, Portland, OR, 97239, USA. morrisoj@ohsu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|December 1, 2017
Summary
This study details a rodent glaucoma model using hypertonic saline injection to induce chronic high intraocular pressure (IOP), aiding optic nerve injury research and neuroprotective treatment testing.
Area of Science:
- Ophthalmology
- Neuroscience
- Animal Models
Background:
- Glaucoma research requires reliable methods to study optic nerve injury.
- Animal models are crucial for understanding disease mechanisms and testing therapies.
Purpose of the Study:
- To outline a method for creating chronic elevation of intraocular pressure (IOP) in rodents.
- To provide a reproducible glaucoma model for studying optic nerve damage.
- To offer a platform for evaluating potential neuroprotective treatments.
Main Methods:
- Obstruction of aqueous humor outflow via hypertonic saline injection into the anterior chamber angle.
- Utilizing a plastic ring to localize sclerosant to the limbus.
- Employing a glass microneedle to inject hypertonic saline into episcleral aqueous outflow veins, targeting Schlemm's canal and trabecular meshwork.
Main Results:
- Gradual inflammation and scarring of the anterior chamber angle.
- Achieved chronic elevation of intraocular pressure (IOP) approximately 1 week post-injection.
- Established a reproducible rodent model for glaucoma research.
Conclusions:
- The described method provides a reliable technique for inducing chronic IOP elevation in rodents.
- This model serves as a valuable tool for investigating glaucoma pathogenesis and testing neuroprotective strategies.
