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Related Concept Videos

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Updated: Feb 17, 2026

Fluorescence detection methods for microfluidic droplet platforms
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A low-cost method for visible fluorescence imaging.

Crissy L Tarver1, Marc Pusey1

  • 1Department of Biological Science, University of Alabama in Huntsville, Huntsville, AL 35899, USA.

Acta Crystallographica. Section F, Structural Biology Communications
|December 5, 2017
PubMed
Summary
This summary is machine-generated.

A low-cost method using smartphone macro lenses effectively images trace fluorescently labeled protein crystals in crystallization plates. This technique aids in screening crystallization conditions for diffraction-quality crystal growth.

Keywords:
fluorescencelow-cost imagingprotein crystallizationtrace fluorescent labeling

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Area of Science:

  • Biophysics
  • Structural Biology
  • Crystallography

Background:

  • Screening crystallization conditions is crucial for obtaining diffraction-quality crystals.
  • Automated imaging systems for crystal screening are prohibitively expensive for many labs.
  • A cost-effective imaging solution is needed to assess crystallization plates.

Purpose of the Study:

  • To develop and validate a simple, inexpensive method for imaging trace fluorescently labeled protein crystals.
  • To demonstrate the feasibility of using smartphone macro lens attachments for crystal imaging.
  • To provide an accessible alternative to expensive automated screening systems.

Main Methods:

  • Trace fluorescent labeling of proteins (β-lactoglobulin B, trypsin, Concanavalin A) using Cascade Yellow, Carboxyrhodamine 6G, and Pacific Blue probes.
  • Imaging of crystallization plates using smartphones (iPhone 6S) with a 10× macro lens attachment (AUKEY Ora).
  • Utilizing LED or laser diodes for excitation and emission filters for fluorescence detection.

Main Results:

  • High-quality images of fluorescently labeled protein crystals were obtained using the smartphone-based system.
  • The method successfully imaged crystals labeled with different fluorescent probes and two-color fluorescence.
  • The cost of the developed system is as low as $50.

Conclusions:

  • Smartphone-based macro imaging offers a cost-effective and accessible solution for screening protein crystallization.
  • This approach can significantly lower the barrier to entry for structural biology research.
  • The technique is adaptable for use with standard microscopy systems for crystal observation.