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A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
Published on: August 7, 2017
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The human kidney capsule contains a functionally distinct mesenchymal stromal cell population
Daniëlle G Leuning1, Marten A Engelse1, Ellen Lievers1
1Department of Internal Medicine, division of Nephrology, Einthoven laboratory of Vascular and Regenerative Medicine; Leiden University Medical Centre, Leiden, The Netherlands.
Plos One
|December 6, 2017
Summary
Human kidney capsule stromal cells (cMSCs) are a novel cell type distinct from kidney perivascular stromal cells (kPSCs). While similar in some aspects, cMSCs exhibit unique properties impacting kidney function and repair.
Area of Science:
- Nephrology
- Stem Cell Biology
- Regenerative Medicine
Background:
- Adult human kidney cortex contains kidney perivascular stromal cells (kPSCs) crucial for kidney repair and function.
- Kidney capsule stromal cells are vital for kidney organization during development and maintain barrier function.
- The distinct anatomical location suggests potential unique properties of kidney capsule stromal cells.
Purpose of the Study:
- To isolate and characterize human kidney capsule mesenchymal stromal cells (cMSCs).
- To compare the properties and functions of cMSCs with kidney perivascular stromal cells (kPSCs).
- To investigate the potential of cMSCs in kidney repair and homeostasis.
Main Methods:
- Isolation of cMSCs from human cadaveric kidneys.
- Comparative analysis of cMSCs and kPSCs, including marker expression, mRNA/miRNA profiles, and functional assays.
- Assessment of vascular plexus formation, myofibroblast transformation resistance, immunosuppressive capacity, and HGF production.
Main Results:
- cMSCs share similarities with kPSCs, including support for vascular plexus formation and resistance to myofibroblast transformation.
- Distinct mRNA and miRNA expression profiles were observed between cMSCs and kPSCs.
- cMSCs demonstrated enhanced immunosuppressive capacity but reduced HGF production compared to kPSCs, impairing kidney epithelial repair.
- cMSCs represent a distinct population of human kidney-derived MSCs.
Conclusions:
- Capsule mesenchymal stromal cells (cMSCs) are a novel, distinct human kidney-derived mesenchymal stem cell (MSC) population.
- Phenotypically similar stromal cells exhibit significant functional diversity based on their anatomical origin within the kidney.
- These findings highlight the importance of stromal cell heterogeneity in kidney physiology and disease.
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