A new method for non-invasive biomass determination based on stereo photogrammetry

Area of Science:

• Plant Biology
• Biotechnology
• Biomass Estimation

Background:

• Accurate biomass estimation is crucial for understanding plant growth dynamics.
• Current methods for biomass assessment can be destructive or labor-intensive.
• Developing non-destructive techniques is essential for longitudinal studies.

Purpose of the Study:

• To develop and validate a novel, non-destructive photogrammetric method for estimating biomass in biological samples cultured in dishes.
• To assess the accuracy of the developed method by correlating volumetric data with experimental measurements.
• To demonstrate the utility of the method for calculating specific growth rates (µmax).

Main Methods:

• Construction of a photogrammetric system including a DSLR camera, illuminated rotating platform, and Arduino control.
• Development of stereo photogrammetry software for 3D sample reconstruction.
• Acquisition of image data over 14 days for plant tissue cultures (Salvia fruticosa and Ocimum basilicum calli).

Main Results:

• Successful 3D reconstruction and volumetric data acquisition of plant calli.
• High correlation between photogrammetric volumetric data and experimental measurements.
• Accurate calculation of specific growth rates (µmax) for S. fruticosa (0.14 day⁻¹) and O. basilicum (0.16 day⁻¹).

Conclusions:

• The developed non-destructive photogrammetric method provides accurate biomass estimation for plant cultures.
• This approach enables precise calculation of growth rates, facilitating biological studies.
• Photogrammetry offers a promising, high-potential tool for applications in the biological sciences.