Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Estimating single-cell elastic modulus in a serial microfluidic cytometer from time-of-flight and fluorescence signals analysis.

Lab on a chipĀ·2026
Same author

Identifying Immunomodulatory Subpopulations of Adipose Stromal Vascular Fraction and Stem/Stromal Cells Through Single-Cell Transcriptomics and Bulk Proteomics.

Stem cell reviews and reportsĀ·2025
Same author

Selection of Force Sensors for <i>In Situ</i> Measurement of Neotissue Microenvironments.

Tissue engineering. Part AĀ·2024
Same author

Microparticles with tunable, cell-like properties for quantitative acoustic mechanophenotyping.

Microsystems & nanoengineeringĀ·2023
Same author

Chondrogenesis of Adipose-Derived Stem Cells Using an Arrayed Spheroid Format.

Cellular and molecular bioengineeringĀ·2022
Same author

Single-Cell Microgels for Diagnostics and Therapeutics.

Advanced functional materialsĀ·2022
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNĪ”7 truncated protein.

BioTechniquesĀ·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniquesĀ·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniquesĀ·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniquesĀ·2026
Same journal

Why and when should we choose digital PCR?

BioTechniquesĀ·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniquesĀ·2026
See all related articles

Related Experiment Video

Updated: Feb 17, 2026

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
07:23

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Published on: September 26, 2013

42.6K

Processing fixed and stored adipose-derived stem cells for quantitative protein array assays.

Jessica S Sadick1, Eric M Darling1,2,3,4

  • 1Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI.

Biotechniques
|December 14, 2017
PubMed
Summary
This summary is machine-generated.

This study enhances the FITSAR method for analyzing proteins in fixed cells, enabling multiplex protein detection in long-term stored samples like adipose-derived stem cells (ASCs). This improves cellular subpopulation characterization for biological research.

Keywords:
FITSARmultiplex ELISAparaformaldehydeprotein characterization

More Related Videos

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
09:20

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

30.2K
Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
05:57

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

4.0K

Related Experiment Videos

Last Updated: Feb 17, 2026

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
07:23

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Published on: September 26, 2013

42.6K
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
09:20

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

30.2K
Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation
05:57

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Published on: December 30, 2021

4.0K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Proteomics

Background:

  • Accurate characterization of cellular subpopulations is crucial for understanding normal and pathological biological mechanisms.
  • Cell fixation is a common sample preparation technique but presents challenges for subsequent protein analysis.
  • The previously established FITSAR method allows protein isolation and characterization from fixed, enriched cell subpopulations.

Purpose of the Study:

  • To improve the FITSAR method for compatibility with sensitive multiplex protein arrays.
  • To demonstrate the applicability of the enhanced FITSAR method to long-term stored samples.
  • To enable robust protein analysis from fixed cells for improved biological insights.

Main Methods:

  • Enhancement of the formaldehyde-fixed intracellular target-sorted antigen retrieval (FITSAR) method.
  • Integration of the FITSAR method with highly sensitive multiplex protein arrays.
  • Application of the FITSAR method to enzyme-linked immunosorbent assay (ELISA) panels for protein detection.

Main Results:

  • Demonstrated compatibility of the improved FITSAR method with multiplex protein arrays.
  • Successfully applied the method to human adipose-derived stem cells (ASCs) stored for up to 1 month.
  • Enabled parallel detection of cell adhesion molecules (CAMs) in fixed and stored ASCs.

Conclusions:

  • The enhanced FITSAR method facilitates sensitive multiplex protein analysis from fixed and long-term stored cell samples.
  • This advancement supports more comprehensive characterization of cellular subpopulations.
  • The improved method has significant implications for biological research and diagnostics.