Modifications in routine protocol of RNA isolation can improve quality of RNA purified from adipocytes
Maxim Yu Sinitsky1, Vera G Matveeva2, Maxim A Asanov2
1Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, Kemerovo, 650002, Russia; The Federal Research Center of Coal and Coal Chemistry of SB RAS, Leningradsky Avenue 10, Kemerovo, 650065, Russia.
Abstract:
Adipose tissue is of interest in the context of its role in the pathogenesis of cardiovascular diseases. Modern experimental techniques require a well-purified RNA, but all the routine protocols for RNA extraction have a number of limitations in case of fatty tissues. Here we described a modified protocol for RNA extraction from human adipocytes based on routine column method. Suggested modifications optimized the sample preparation, lysis and washing lead to enhance RNA purity. We conclude that the current protocol for total RNA purification from adipocytes allows extracting a high-quality RNA devoid of fatty acids, organic solvents and salts contamination.
