Simplified adaptor for stereotactic surgery in non-human primates.

Area of Science:

• Neuroscience
• Surgical Technology
• Primate Research

Background:

• Stereotactic procedures in non-human primates (NHPs) are complex, often requiring specialized equipment and expertise.
• Current methods rely on the ear-bar zero (EBZ) reference, which is not directly visible during surgery.
• High-cost MRI/CT imaging and intricate computer processing are typically needed, limiting accessibility for many researchers.

Purpose of the Study:

• To develop a simplified, cost-effective adaptor for NHP stereotactic surgery.
• To facilitate easier identification of the EBZ reference point.
• To enable precise targeting without reliance on advanced imaging or computational methods.

Main Methods:

• A novel adaptor was designed, incorporating a coronal tilt arc, an electrode/cannula carrier, and an anchor for conventional stereotactic frames.
• An anchor notch was integrated for straightforward EBZ identification.
• Digital distance sensors were included, obviating the need for imaging data or complex computer processing.

Main Results:

• The system demonstrated sub-millimeter accuracy in phantom targeting tests.
• Successful staining of deep brain structures was achieved using methylene blue infusion.
• The adaptor simplifies EBZ setting and streamlines targeting, akin to small animal procedures.

Conclusions:

• This simplified adaptor significantly lowers the barrier to entry for NHP stereotactic surgery.
• It reduces the reliance on expensive imaging and specialized training.
• The system empowers a broader range of researchers to conduct complex NHP experiments.