Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Toxicity Testing in Animals01:23

Toxicity Testing in Animals

16
Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
16

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metallothionein-Inspired Dual-Stage Ion-Regulatory Coatings With Infection-Triggered Bactericidal Activity and Long-Term Antifouling Protection.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

An intelligent MRI-based all-in-one diagnostic strategy for axillary lymph node status in breast cancer.

Nature communications·2026
Same author

Analysis of in vitro profiling data of cosmetic ingredients within the Tox21 10K compound library for bioactivity and potential toxicity.

BMC pharmacology & toxicology·2026
Same author

Scalable hypothalamic neuron differentiation from human pluripotent stem cells suitable for modeling metabolic disorders.

Stem cell reports·2026
Same author

Characterizing Antibody-Drug Conjugates with High Molecular Heterogeneity by Native Direct Mass Technology.

Analytical chemistry·2026
Same author

(2R,6R)-HNK improved LPS-induced depression-like behavior by inhibiting Vcam1/Caspase-1/IL-1β pathway.

International immunopharmacology·2026
Same journal

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026
Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026
Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026
Same journal

Hierarchy of MS-Based Evidence.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Exosomes from HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinoma: Selective Cargo Packaging.

Journal of proteome research·2026
See all related articles

Related Experiment Video

Updated: Feb 16, 2026

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

13.2K

Omics-Based Platform for Studying Chemical Toxicity Using Stem Cells.

Yan Han1, Jinghua Zhao2, Ruili Huang2

  • 1Newomics Inc. , Emeryville, California 94608, United States.

Journal of Proteome Research
|December 21, 2017
PubMed
Summary
This summary is machine-generated.

New toxicity testing uses stem cells and high-throughput screening to predict chemical responses. Chemical toxicity varies by stem cell type and differentiation stage, offering mechanistic insights for safety evaluations.

Keywords:
LC-MSLOPACcantharidinchemical toxicityidarubicinproteomicsqHTSstem cells

More Related Videos

A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans
09:01

A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans

Published on: March 14, 2019

7.7K
Developmental Toxicity Assay Based on Real-Time Monitoring of Fibroblast Growth Factor Signal Disruption in Human Induced Pluripotent Stem Cells
05:45

Developmental Toxicity Assay Based on Real-Time Monitoring of Fibroblast Growth Factor Signal Disruption in Human Induced Pluripotent Stem Cells

Published on: October 10, 2025

556

Related Experiment Videos

Last Updated: Feb 16, 2026

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

13.2K
A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans
09:01

A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans

Published on: March 14, 2019

7.7K
Developmental Toxicity Assay Based on Real-Time Monitoring of Fibroblast Growth Factor Signal Disruption in Human Induced Pluripotent Stem Cells
05:45

Developmental Toxicity Assay Based on Real-Time Monitoring of Fibroblast Growth Factor Signal Disruption in Human Induced Pluripotent Stem Cells

Published on: October 10, 2025

556

Area of Science:

  • Toxicology
  • Stem Cell Biology
  • High-Throughput Screening

Background:

  • Traditional chemical toxicity testing relies on animal models, which have limitations in predicting human responses.
  • In vitro assays using human stem cells offer a more physiologically relevant alternative for toxicity assessment.
  • Quantitative high-throughput screening (qHTS) enables rapid, large-scale screening of chemical compounds.

Purpose of the Study:

  • To develop and validate a novel stem cell-based assay system for chemical toxicity testing.
  • To identify molecular mechanisms underlying chemical toxicity using omics technologies.
  • To predict in vivo chemical responses based on in vitro findings.

Main Methods:

  • Established a two stem cells-two lineages assay system using human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs).
  • Performed qHTS phenotypic screening of the LOPAC1280 library.
  • Conducted mechanistic studies using proteomics and bioinformatics on selected chemical treatments.

Main Results:

  • Identified 38 preliminary chemical toxicity hits in hMSCs.
  • Demonstrated that chemical toxicity is dependent on stem cell type and differentiation stage, with hiPSCs generally being more sensitive.
  • Proteomics identified over 3000 proteins, and bioinformatics revealed apoptosis and G2/M as key pathways for idarubicin toxicity.

Conclusions:

  • Stem cell-based assays coupled with qHTS provide valuable mechanistic insights into chemical toxicity.
  • This approach can help prioritize chemicals for further toxicological evaluation, improving safety assessments.
  • The findings highlight the importance of considering cell type and differentiation state in toxicity testing.