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Cytocompatibility of a self-adhesive gutta-percha root-filling material
Joyce Nascimento1, Miriam Zaccaro Scelza1, Gutemberg Gomes Alves1
1Department of Cell and Molecular Biology, Fluminense Federal University, Niterói, Brazil.
Context:
A novel root-filling material based on the incorporation of ultrafine alkaline bioactive glass particles (bioactive gutta-percha, [BGP]) was developed to work without sealer.
Aim:
In the present study, the objective was to verify the in vitro biological response to this material by assessing its cytocompatibility.
Materials And Methods:
Prototypes of BGP were compared to conventional gutta-percha (GP), dense polystyrene beads as a negative control and fragments of latex as a positive control. Extracts of each material were prepared according to ISO 10993-5:2009, and human osteoblast-like cells in primary culture were exposed to all extracts for 24 h. Cell viability was assayed sequentially for three different parameters: mitochondrial activity, membrane integrity, and cell density.
Statistical Analysis Used:
Nonparametric analysis (using Kruskal-Wallis test combined with post hoc Dunn's test) was performed for comparison among groups, with significance established at 5%.
Results:
BGP reduced mitochondrial activity to 62% of control, but presented no toxicity on membrane integrity and proliferation assays. BGP effect on metabolism was dose-dependent and reduced to acceptable levels with dilution.
Conclusion:
The novel GP material presented slight dose-dependent effects on cell metabolism but did not affect cell survival.
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