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Updated: Feb 16, 2026

Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
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Organoids as a Model to Study Infectious Disease.

Kristen A Engevik1, Andrea L Matthis1, Marshall H Montrose1

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Methods in Molecular Biology (Clifton, N.J.)
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Summary

Gastric organoid cultures mimic stomach tissue in the lab. Researchers detail methods for growing these organoids and infecting them with Helicobacter pylori to study cellular responses to infection.

Keywords:
Fluorescent dyeGastric organoidsHelicobacter pyloriMicroinjectionPathogens

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Area of Science:

  • Gastroenterology and Cell Biology
  • Infectious Disease Research
  • 3D Cell Culture Models

Background:

  • Gastric organoids offer a novel in vitro model for native gastric epithelial tissue.
  • These three-dimensional structures are derived from epithelial stem cells.
  • They serve as a valuable tool for investigating host physiology.

Purpose of the Study:

  • To outline laboratory protocols for cultivating gastric organoids.
  • To describe the microinjection technique for introducing pathogens into gastric organoids.
  • To facilitate the study of cellular responses to infection within a controlled environment.

Main Methods:

  • Isolation and culture of gastric epithelial stem cells.
  • Development of three-dimensional gastric organoid structures.
  • Microinjection of Helicobacter pylori into the organoid lumen.

Main Results:

  • Successful establishment of gastric organoid cultures.
  • Demonstration of pathogen microinjection into organoid lumens.
  • Observation of cellular responses to Helicobacter pylori infection.

Conclusions:

  • Gastric organoid systems provide a robust platform for studying host-pathogen interactions.
  • Established protocols enable the investigation of cellular mechanisms underlying gastric infections.
  • This model system advances research into gastric diseases and physiology.