Related Experiment Video
Updated: Feb 16, 2026
10:18
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
Published on: September 19, 2011
26.0K
Engineering Bioinspired Antioxidant Materials Promoting Cardiomyocyte Functionality and Maturation for Tissue
Parvaiz A Shiekh1, Anamika Singh1, Ashok Kumar1
1Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur , Kanpur-208016, Uttar Pradesh, India.
ACS Applied Materials & Interfaces
|January 6, 2018
Summary
This study developed an antioxidant polyurethane (PUAO) to combat oxidative stress in tissue engineering. PUAO effectively reduced cell damage and supported functional cardiac tissue development, showing promise for treating conditions like cardiovascular disease.
Area of Science:
- Biomaterial Science
- Tissue Engineering
- Cardiovascular Research
Background:
- Oxidative stress is implicated in various diseases, including myocardial infarction and chronic wounds.
- Antioxidant polymers offer a novel strategy to mitigate oxidative stress in regenerative medicine.
Purpose of the Study:
- To fabricate and evaluate an elastomeric antioxidant polyurethane (PUAO) for tissue engineering.
- To assess PUAO's efficacy in reducing oxidative stress and supporting cardiac cell function.
Main Methods:
- Fabrication and characterization of PUAO.
- In vitro oxidative stress and simulated ischemia-reperfusion models using H9C2 cardiomyocytes.
- Cytotoxicity, degradation, and antioxidant analyses.
- Culturing neonatal rat cardiomyocytes on PUAO films.
Main Results:
- PUAO significantly reduced intracellular oxidative stress and reactive oxygen species (ROS)-induced cell death in cardiomyocytes.
- PUAO rescued cells from hypoxia-induced death under simulated ischemic conditions.
- Cardiomyocytes cultured on PUAO exhibited synchronous beating and expressed key cardiac-specific proteins, indicating functional maturation.
Conclusions:
- PUAO demonstrates significant antioxidant properties and biocompatibility for tissue engineering.
- This biomaterial holds potential for developing scaffolds to treat conditions characterized by oxidative stress, such as cardiovascular diseases and chronic wounds.
Related Concept Videos
00:49What is Genetic Engineering?
80.4K
Overview
80.4K
01:10Heat Engines
3.7K
A heat engine is a device used to extract heat from a source and then convert it into mechanical work used for various applications. For example, a steam engine on an old-style train can produce the work needed for driving the train.
Whenever we consider heat engines (and associated devices such as refrigerators and heat pumps), we do not use the standard sign convention for heat and work. For convenience, we assume that the symbols Qh, Qc, and W represent only the amounts of heat transferred...
Whenever we consider heat engines (and associated devices such as refrigerators and heat pumps), we do not use the standard sign convention for heat and work. For convenience, we assume that the symbols Qh, Qc, and W represent only the amounts of heat transferred...
3.7K
01:20Internal Combustion Engine
2.8K
The internal combustion engine is a heat engine that uses the byproducts of combustion as the working fluid instead of using a heat transfer medium to transfer heat. The combustion is done in a way that produces high-pressure combustion products that can be expanded through a turbine or piston to create work. Internal combustion engines can again be categorized into three kinds: (1) spark ignition gasoline engines, most commonly used in automobiles, (2) compression ignition diesel engines that...
2.8K
02:40The Eukaryotic Promoter Region
19.0K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
19.0K
01:23Epithelial Tissues and Their Functions
41.4K
Epithelial tissues are large sheets of cells covering all of the surfaces of the body. These surfaces can be internal or external, for example, skin, airways, the digestive tract, the urinary system, and the reproductive system. Hollow organs and body cavities that do not connect to the body's exterior, including blood vessels and serous membranes, are lined by epithelial tissue known as the endothelium.
Epithelial tissues provide the body's first line of protection from physical,...
Epithelial tissues provide the body's first line of protection from physical,...
41.4K
01:17Functions of Connective Tissues
17.5K
Connective tissues perform a broad range of functions in the body. Their primary function is to connect and link different tissues in the body and act as packaging material between tissues. The areolar tissue, a connective tissue prototype, commonly cements various tissue types in diverse body organs. In contrast, adipose tissue cushions internal organs while insulating the body from heat loss.
Hard connective tissues, such as bones and cartilage, provide structure and support to the body.
Hard connective tissues, such as bones and cartilage, provide structure and support to the body.
17.5K