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Updated: Feb 16, 2026

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
Published on: September 2, 2014
A method for high transfection efficiency in THP-1 suspension cells without PMA treatment
Xiaoren Tang1, Bushra Aljahdali1, Mansour Alasiri1
1Boston University, Henry M. Goldman School of Dental Medicine, Department of Periodontology, 100E.Newton Street, 02118, Boston, MA, United States.
Abstract:
Adherent cells such as mouse RAW cells or human cancer U2OS cells are beneficial to DNA transfection, with 20%-60% transfection efficiency. However, this DNA transfection is rarely used on suspension cells due to its low transfection efficiency (≤5%). We recently found a new DNA transfection method to increase the efficiency up to 13.5% in suspension cells without PMA treatment. We also found that DNA transfection of human TNFAIP1 or CXCL1 recombinant plasmid DNA in THP-1 cells induces a high level of TNF-α protein. Overall, this new method is simple yet efficient and can be used for the overexpression of DNA in suspension cells.
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