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Early pregnancy protein multiplex screening reflects circulating and urinary divergences associated with the

Margarita L Martinez-Fierro1,2, Claudia Castruita-De La Rosa1, Idalia Garza-Veloz1,2

  • 1a Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas , Zacatecas , Mexico.

Hypertension in Pregnancy
|January 9, 2018
PubMed

Insights

Early detection of preeclampsia is possible using specific angiogenic proteins. Measuring levels of proteins like soluble epidermal growth factor receptor (sEGFR) and vascular endothelial growth factor A (VEGF-A) in pregnant women can predict preeclampsia risk.

Area of Science:

  • Reproductive biology and obstetrics.
  • Biomarker discovery and validation.
  • Maternal-fetal medicine.

Background:

  • Preeclampsia is a major cause of maternal and fetal mortality globally.
  • Accurate biomarkers are needed for early preeclampsia detection and improved patient outcomes.
  • Current diagnostic methods require enhancement for predictive capabilities.

Purpose of the Study:

  • To assess the predictive value of 34 angiogenic-related proteins for preeclampsia development.
  • To identify specific protein biomarkers for early identification of preeclampsia risk.
  • To evaluate protein profiles at multiple gestational weeks for predictive accuracy.

Main Methods:

  • A nested case-control study involving pregnant women.
  • Analysis of 34 angiogenic proteins in urine and plasma samples at 12, 16, and 20 gestational weeks.
  • Utilized Bio-Plex Pro™ Human Cancer Biomarker Panels for protein profiling.

Main Results:

  • Significant differences in urine concentrations of sEGFR, HGF, ANG-2, ENG, sFASL, IL-6, PLGF, and VEGF-A at 12 GW.
  • Distinct levels of PRL, ANG-2, TGF-α, and VEGF-A identified at 16 GW.
  • Elevated sIL-6Rα, ANG-2, and sFASL concentrations at 20 GW were associated with preeclampsia.

Conclusions:

  • Multiple angiogenic proteins, including sEGFR, HGF, ANG-2, sFASL, IL-6, PLGF, VEGF-A, PRL, TGF-α, FGF-b, sHER2/Neu, sIL-6Rα, ENG, uPA, and IGFBP-1, are predictive of preeclampsia.
  • These proteins show potential as early diagnostic markers for preeclampsia.
  • Further consideration of these biomarkers for clinical use is warranted.
Abstract

Keywords:
Angiogenesisbiomarkerscancerplacentapreeclampsiascreeningurine

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