Usefulness of the CLLflow score

Area of Science:

• Hematology
• Immunophenotyping
• Flow Cytometry

Background:

• B-cell lymphoproliferative disorders (B-LPD) require accurate diagnosis and classification.
• The Moreau score (MS) is a current method, but improvements are sought.
• The CLLflow score is proposed as a more effective diagnostic tool.

Purpose of the Study:

• To evaluate the utility of the CLLflow score in diagnosing B-cell lymphoproliferative disorders.
• To compare the CLLflow score's performance against the Moreau score in cases with inconclusive immunophenotypes.

Main Methods:

• The CLLflow score was determined in peripheral blood or bone marrow samples.
• Cases included those with MS of 3 (n=52) and CD5-positive score of 2 (n=38).
• Control groups included B-LPD with MS 0-1 (n=95), CD5-negative score 2 (n=24), and CLL (MS 4-5, n=166).

Main Results:

• The CLLflow score correctly identified all chronic lymphocytic leukemia (CLL) cases.
• It was negative in all MS <2 cases, irrespective of CD200 expression.
• The CLLflow score showed positivity in 71% of MS 3, 29% of CD5-positive score 2, and 8% of CD5-negative score 2 samples.

Conclusions:

• The CLLflow score demonstrates utility in the differential diagnosis of B-cell lymphoproliferative disorders.
• It is particularly valuable for cases with inconclusive immunophenotypes.
• This score offers an improved approach compared to the Moreau score in specific diagnostic scenarios.