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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
Published on: May 8, 2016
Isolation and Characterization of Mouse Intrahepatic Lymphocytes by Flow Cytometry
Florian Wiede1,2, Tony Tiganis3,4
1Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC, Australia. Florian.Wiede@monash.edu.
Abstract:
In the field of cellular immunology multicolor flow cytometry is a frequently applied method that allows for the simultaneously detection of multiple parameters on an individual cell basis. Flow cytometry can be used to characterize a wide range of immune cell subsets using fluorophore-conjugated antibodies to a wide range of cellular antigens. The isolation of immune cells from nonlymphoid tissue and their preparation for flow cytometry can be a challenging process with respect to immune cell yields and viability. Here we describe a method for the efficient isolation of viable mouse intrahepatic lymphocytes (IHL) from normal liver tissue and liver cancer and their subsequent characterization by multicolor flow cytometry.
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