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Published on: January 3, 2017
Ultrafast determination of vitamin E using LC-ESI-MS/MS for preclinical development of new nutraceutical formulations
Noemi Villaseca-González1, Virginia Rodríguez Robledo1, Lucía Castro-Vázquez1
1Faculty of Pharmacy, University of Castilla-La Mancha, Albacete, Spain.
Aim:
We proposed a rapid and high quality method to determine α-tocopherol (α-T) in different biopharmaceutical samples using liquid chromatography-diode array detector on-line ESI-MS/MS.
Materials & Methods:
A working standard solution of α-T and internal standard, phenyl-5,7-dimethyl-d6-α-tocopherol, were used for optimization and validation of the method. Levels of α-T in nanoemulsions, serum and plasma samples were evaluated.
Results & Conclusion:
Precision (1% for retention time, 5% for peak area and 3% for relative peak area), linearity range (among 0.625-20.0 μg ml-1), LOD and LOQ, accuracy and matrix effect were studied. The validated chromatographic method is presented as valuable analytical tool for the determination of α-tocopherol in loaded drug delivery systems and in biodistribution levels in blood samples.
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