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Paper-Based DNA Reader for Visualized Quantification of Soil-Transmitted Helminth Infections.

Alex Guan Wang, Tianyu Dong, Hayam Mansour1

  • 1Department of Cell Biology, National Research Center , Cairo, Egypt , 12622.

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|January 17, 2018
PubMed
Summary
This summary is machine-generated.

A new paper-based diagnostic device, the quantitative paper-based DNA reader (qPDR), enables portable and inexpensive molecular quantification of soil-transmitted helminth (STH) infections. This method successfully detected STH genetic markers in children from rural Honduras.

Keywords:
distance-based detectionequipment-free quantificationnucleic acid testingpaper-based analytical devicepoint-of-caresoil-transmitted helminth

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Area of Science:

  • Biomedical Diagnostics
  • Molecular Biology
  • Parasitology

Background:

  • Soil-transmitted helminth (STH) infections affect one-third of the global population, particularly in resource-limited regions.
  • Accurate STH diagnosis requires portable, inexpensive, and user-friendly analytical tools.
  • Current diagnostic methods often lack the necessary portability and affordability for widespread use in endemic areas.

Purpose of the Study:

  • To develop a novel, portable, and quantitative diagnostic device for molecular detection of STH.
  • To establish a distance-based readout system for DNA quantification, eliminating the need for external instruments.
  • To validate the device's efficacy in detecting STH genetic markers in a real-world setting.

Main Methods:

  • Introduction of the quantitative paper-based DNA reader (qPDR), a paper-based device utilizing SYBR Green I and cellulose interactions.
  • Development of a distance-based quantification method for double-stranded DNA.
  • Integration of qPDR with smartphone-controlled portable polymerase chain reaction (PCR) thermal cycler.
  • Application of the integrated system to detect genetic markers of Trichuris trichiura in patient samples.

Main Results:

  • The qPDR device achieved distance-based quantification of DNA in as little as 6 minutes.
  • The system demonstrated the ability to quantify minute amounts of STH genetic markers.
  • Successful detection of Trichuris trichiura genetic markers from expelled adult worms in children treated for STH in Honduras.

Conclusions:

  • The qPDR offers a promising, low-cost, and portable solution for molecular diagnosis of STH infections.
  • This technology can significantly improve STH surveillance and diagnosis in resource-limited settings.
  • The developed diagnostic approach holds potential for broader applications in molecular diagnostics for infectious diseases.