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Cardiac MRI in Small Animals.

Min-Chi Ku1, Till Huelnhagen1, Thoralf Niendorf1,2

  • 1Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|January 18, 2018
PubMed
Summary
This summary is machine-generated.

This study details a step-by-step cardiac magnetic resonance (MR) imaging protocol for mice, enabling precise in vivo diagnosis of heart defects. The method captures cardiac function and provides key morphometric and functional parameters for research.

Keywords:
Cardiac magnetic resonance imaging (CMR)FunctionHeartMagnetic resonance imaging (MRI)Mouse

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Area of Science:

  • Cardiovascular Imaging
  • Preclinical Research
  • Medical Diagnostics

Background:

  • Cardiac magnetic resonance (MR) imaging is crucial for diagnosing and predicting heart defects in vivo.
  • Accurate assessment of cardiac structure and function is vital in preclinical models.
  • Existing protocols may require refinement for detailed mouse cardiac analysis.

Purpose of the Study:

  • To provide a comprehensive, step-by-step protocol for cardiac MR imaging in mice.
  • To enable precise in vivo assessment of cardiac contractile function and morphology.
  • To establish a foundation for adapting the protocol to other small animal models, such as rats.

Main Methods:

  • Detailed step-by-step protocol for performing cardiac MR imaging in mice.
  • Acquisition of a series of MR images to capture heart contractility.
  • Post-processing of image data to derive quantitative morphometric and functional parameters.

Main Results:

  • The protocol yields precise morphometric parameters including myocardial mass, wall thickness, and ventricular volumes (end-systolic and end-diastolic).
  • Functional parameters such as stroke volume and ejection fraction are accurately determined.
  • The imaging technique allows for in vivo evaluation of cardiac contractile function.

Conclusions:

  • This detailed cardiac MR imaging protocol offers a robust method for in vivo cardiac assessment in mice.
  • The protocol facilitates accurate diagnosis and prognosis of cardiac defects in preclinical studies.
  • Adaptations for larger animals like rats are feasible, expanding the utility of the technique.