Related Experiment Video
Updated: Feb 15, 2026

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
Published on: March 13, 2013
Bifunctional Fluorescent Probe for Sequential Sensing of Thiols and Primary Aliphatic Amines in Distinct Fluorescence
Meiyu Huang1, Lingliang Long1, Ning Wang1
1School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, P.R. China.
Abstract:
Thiols and primary aliphatic amines (PAA) are ubiquitous and extremely important species in biological systems. They perform significant interplaying roles in complex biological events. A single fluorescent probe differentiating both thiols and PAA can contribute to understanding the intrinsic inter-relationship of thiols and PAA in biological processes. Herein, we rationally constructed the first fluorescent probe that can respond to thiols and PAA in different fluorescence channels. The probe exhibited a high selectivity and sensitivity to thiols and PAA. In addition, it displayed sequential sensing ability when the thiols and PAA coexisted. The application experiments indicated that the probe can be used for sensing thiols and PAA in human blood serum. Moreover, the fluorescence imaging of endogenous thiols and PAA as well as antihypertensive drugs captopril and amlodipine in living cells were successfully conducted.
Related Concept Videos
Basicity of Aliphatic Amines
To measure the basicity of amines, two conventions are generally used. The first defines Kb as the basicity constant for the deprotonation reaction of water by the amine, as presented in Figure 1. Conventionally, lower Kb indicates higher...
Nomenclature of Primary Amines
Preparation and Reactions of Thiols
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Atomic Fluorescence Spectroscopy
Confocal Fluorescence Microscopy

