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Updated: Aug 18, 2026

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
Published on: October 20, 2018
Detection of protein targets with a single binding epitope using DNA-templated photo-crosslinking and strand
Yiran Huang1, Wenlu Zheng2, Xiaoyu Li1
1Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.
Abstract:
DNA-based probes are powerful analytical tools for protein detection and analysis. Target-induced DNA assembly is a widely used strategy to transduce target-ligand binding to detectable signals. However, most of the existing methods based on DNA assembly require two or more binding sites on the target protein. Here we report a novel detection method suitable for protein targets with just a single binding site. This method is based on target-induced probe assembly, DNA-templated photo-crosslinking, and DNA-mediated toehold strand displacement to form a tri-probe complex that is specific for target protein.
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