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Related Experiment Video

Updated: Feb 15, 2026

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Published on: February 25, 2021

Spotiton: New features and applications.

Venkata P Dandey¹, Hui Wei¹, Zhening Zhang¹

¹The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY, USA.

Journal of Structural Biology

|January 26, 2018

Summary

This summary is machine-generated.

Spotiton, a new instrument for cryo-electron microscopy (cryoEM) sample vitrification, shows no negative impact on sample integrity. It is now routinely used for diverse sample types, achieving high-resolution reconstructions.

Keywords:

cryoEM vitrification nanowire grids piezo dispensing

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Area of Science:

Biophysics
Structural Biology
Microscopy

Background:

Cryo-electron microscopy (cryoEM) requires high-quality vitrified samples for high-resolution structure determination.
Sample preparation is a critical bottleneck in cryoEM workflows.
Novel instrumentation is needed to improve the efficiency and reproducibility of sample vitrification.

Purpose of the Study:

To present updates on the features and applications of the Spotiton instrument.
To evaluate the performance of Spotiton for sample vitrification in cryoEM.
To demonstrate the utility of Spotiton for routine use with diverse biological samples.

Main Methods:

Utilized the Spotiton instrument for automated sample vitrification.
Prepared test specimens using the Spotiton system.
Performed single particle analysis (SPA) on vitrified samples.
Assessed sample integrity and resolution achievable by cryoEM.

Main Results:

Spotiton successfully vitrified a wide variety of biological samples.
Test specimens prepared with Spotiton were reconstructed to approximately 3 Å resolution using routine SPA.
The vitrification process using Spotiton showed no apparent deleterious effect on sample integrity.
The Spotiton system is now in routine and continuous use in the laboratory.

Conclusions:

Spotiton is a reliable and effective instrument for cryoEM sample vitrification.
The instrument facilitates high-resolution structure determination without compromising sample integrity.
Spotiton enables routine and versatile application for diverse sample types in cryoEM research.