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Laxative effect of repeated Daiokanzoto is attributable to decrease in aquaporin-3 expression in the colon
Risako Kon1, Miho Yamamura2, Yukari Matsunaga3
1Global Research Center for Innovative Life Science, Hoshi University, Tokyo, Japan.
Journal of Natural Medicines
|January 31, 2018
Summary
Daiokanzoto (DKT) provides a sustained laxative effect for chronic constipation by maintaining aquaporin-3 (AQP3) expression and gut microbiota balance. This indicates DKT is a viable long-term treatment option.
Area of Science:
- Pharmacology
- Gastroenterology
- Microbiology
Background:
- Daiokanzoto (DKT) is a traditional herbal medicine known for its laxative properties, primarily attributed to sennoside A in rhubarb.
- Previous research linked the laxative effect of sennoside A to decreased aquaporin-3 (AQP3) expression in colonic epithelial cells due to inflammation.
- A combination of glycyrrhizin (from licorice) and sennoside A was found to attenuate inflammation and reduce the laxative effect, suggesting potential for chronic constipation treatment.
Purpose of the Study:
- To investigate the sustained laxative effect of repeated Daiokanzoto (DKT) administration.
- To analyze the impact of DKT on aquaporin-3 (AQP3) expression in the colon.
- To evaluate the changes in gut microbiota composition following repeated DKT treatment.
Main Methods:
- Rats were administered DKT or Daio (rhubarb) for 7 days.
- Aquaporin-3 (AQP3) expression in colonic tissues was assessed.
- Gut microbiota composition was analyzed using sequencing techniques.
Main Results:
- Repeated DKT administration resulted in decreased AQP3 expression and diarrhea, similar to single-dose effects.
- Daio treatment alone did not induce these changes.
- DKT maintained gut microbiota homeostasis, while Daio altered specific bacterial families (reduced Lactobacillaceae, Bifidobacteriaceae, Bacteroidaceae; increased Lachnospiraceae).
Conclusions:
- Daiokanzoto (DKT) demonstrates a sustained laxative effect upon repeated administration.
- This sustained effect is likely due to the maintenance of decreased AQP3 expression and gut microbiota homeostasis.
- DKT shows promise as a long-term therapeutic option for managing chronic constipation.
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