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Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction
Diego Morales1, Adriana J Piris1, Alejandro Ruiz-Rodriguez1
1Dept. of Production and Characterization of Novel Foods, Institute of Food Science Research - CIAL (UAM+CSIC), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autonoma de Madrid, Madrid, 28049, Spain.
Biotechnology Progress
|February 2, 2018
Summary
Shiitake mushrooms were sequentially extracted to isolate bioactive compounds for cardiovascular disease (CVD) prevention. This method effectively separated antioxidants, antihypertensive, and cholesterol-lowering compounds from a single batch.
Area of Science:
- Biotechnology
- Food Science
- Pharmacology
Background:
- Cardiovascular diseases (CVDs) represent a significant global health burden.
- Natural products, such as those from Lentinula edodes (shiitake mushrooms), are increasingly investigated for therapeutic potential.
- Efficient extraction of bioactive compounds is crucial for their application.
Purpose of the Study:
- To develop and optimize a sequential extraction method for isolating bioactive compounds from shiitake mushrooms.
- To characterize the separated fractions for their potential in managing cardiovascular diseases.
- To evaluate the efficacy of different extraction techniques in compound isolation.
Main Methods:
- A sequential extraction process combining plain water extraction, supercritical fluid extraction (SFE), and hot water extraction.
- Analysis of extracted fractions for bioactive compounds including ß-glucans, chitins, eritadenine, lenthionine, ergosterol, proteins/peptides, and phenolic compounds.
- Assay of antioxidant activity (ABTS, DPPH), angiotensin-I converting enzyme (ACE) inhibition, and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) inhibition.
Main Results:
- Sequential extraction yielded distinct fractions with varying bioactive compound profiles.
- Water-soluble fractions exhibited significant antioxidant capacity and inhibited ACE and HMGCR.
- SFE extracts contained ergosterol and other lipid-soluble compounds with HMGCR inhibitory activity.
- Dietary fibers were successfully isolated in the final extraction step.
Conclusions:
- A sequential extraction strategy enables selective separation of diverse bioactive compounds from shiitake mushrooms.
- This approach offers a sustainable method for obtaining natural compounds with potential cardiovascular benefits.
- The isolated compounds demonstrate promise for the development of functional foods and pharmaceuticals for CVD management.
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