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Updated: Feb 15, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Published on: August 18, 2012
The EPR Method for Detecting Nitric Oxide in Plant Senescence
1The National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. azsun@sibs.ac.cn.
Abstract:
Nitric oxide (NO) is gaining increasing attention as a central molecule with diverse signaling functions. It has been shown that NO acts as a negative regulator of leaf senescence. In this chapter, we describe a highly selective method, electron paramagnetic resonance ([EPR], also known as electron spin resonance [ESR]), for NO determination in leaf senescence. An iron complex of ferrous and mononitrosyl dithiocarbamate (Fe2+(DETC)2) is used as a chelating agent for NO. Using ethyl acetate as extracting solvent, the NOFe2+(DETC)2 complex is extracted and determined by EPR spectrometer.
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