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Updated: Feb 15, 2026

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Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope
Chun-Lin Shi1, Melinka A Butenko2
1Department of Molecular Biosciences, University of Oslo, Oslo, Norway.
Abstract:
Scanning electron microscope (SEM) is a type of electron microscope which produces detailed images of surface structures. It has been widely used in plants and animals to study cellular structures. Here, we describe a detailed protocol to prepare samples of floral abscission zones (AZs) for SEM, as well as further image analysis. We show that it is a powerful tool to detect morphologic changes at the cellular level during the course of abscission in wild-type plants and to establish the details of phenotypic alteration in abscission mutants.
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