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Updated: Feb 15, 2026

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Published on: November 10, 2009
Molecular characterization and complete genome of a novel nepovirus from red clover
Igor Koloniuk1, Jaroslava Přibylová2, Jana Fránová2
1Department of Plant Virology, Biology Centre, Institute of Plant Molecular Biology, The Czech Academy of Sciences, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic. koloniuk@umbr.cas.cz.
Abstract:
During high throughput sequencing (HTS) of leaves from a symptomatic red clover plant, a new RNA virus, tentatively named red clover nepovirus A (RCNVA), was discovered. The complete genomic sequence was determined and characterized. Particularly noteworthy was that RCNVA shares high sequence identities in RNA1 with a group of phylogenetically related nepoviruses while homologies in the RNA2 segments are markedly lower. Based on the genomic organization and phylogenetic attributes, RCNVA should be classified as a novel virus of the genus Nepovirus (subfamily Comovirinae, family Secoviridae, order Picornavirales).
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