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Bioengineered in vitro models of thrombosis: methods and techniques
Yu Shrike Zhang1, Rahmi Oklu2, Hassan Albadawi2
1Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.
Cardiovascular Diagnosis and Therapy
|February 6, 2018
Summary
Biomimetic in vitro models, called thrombosis-on-a-chip systems, are advancing the study of thrombosis. These human cell-based platforms accurately mimic vascular pathology, aiding research and personalized treatment development.
Area of Science:
- Biomedical Engineering
- Vascular Biology
- Pathophysiology
Background:
- Thrombosis is a common vascular disorder.
- Traditional in vivo animal models have limitations.
- Recent advancements enable the development of in vitro models.
Purpose of the Study:
- To review recent advances in engineering biomimetic in vitro models of thrombosis.
- To highlight the capabilities of thrombosis-on-a-chip systems.
- To discuss their application in studying thrombosis and developing personalized treatments.
Main Methods:
- Engineering of biomimetic in vitro models.
- Integration with microfluidic devices to create thrombosis-on-a-chip systems.
- Utilizing human cells to recapitulate native thrombosis characteristics.
Main Results:
- Thrombosis-on-a-chip systems successfully reproduce key features of native thrombosis.
- These models mimic vascular structures, extracellular matrix properties, and cellular composition.
- They accurately recapitulate thrombosis pathophysiology.
Conclusions:
- Human cell-based thrombosis-on-a-chip platforms are enabling in vitro models for thrombosis research.
- These systems offer a powerful tool to study vascular disorders.
- They facilitate the development of personalized treatment strategies for thrombosis.
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