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Updated: Feb 14, 2026

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Luminol-K3Fe(CN)6 chemiluminescence system for the determination of glipizide
Xin Chen1, Li-Li Xing2, Yu-Hai Tang2
1The 405 Hospital of Hanzhong City, Hanzhong, Shaanxi 723312, China.
Abstract:
A rapid and sensitive flow-injection chemiluminescence (CL) method for the determination of glipizide was developed on the basis of finding that glipizide can enhance the CL intensity of the luminol-K3Fe(CN)6 system. In optimum condition, the increased CL intensity was directly proportional to the concentration of glipizide in the range from 4.0×10-8 g/mL to 1.0×10-6 g/mL and the detection limit was 1.0×10-8 g/mL glipizide. The relative standard deviation (RSD) of the developed method was 2.1% with 11 repeated measurements of 1.0×10-7 g/mL glipizide. The developed method has been successfully applied to the analysis of glipizide in its pharmaceutical preparations.
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