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Updated: Feb 14, 2026

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
Published on: December 29, 2017
Reading cytosine modifications within chromatin
Elise A Mahé1, Thierry Madigou1, Gilles Salbert1
1a University of Rennes 1, UMR6290 CNRS , Institute of Genetics and Development of Rennes , Campus de Beaulieu, Rennes Cedex , France.
Abstract:
Zinc-finger and homeodomain transcription factors have been shown in vitro to bind to recognition motifs containing a methylated CpG. However, accessing these motifs in vivo might be seriously impeded by the inclusion of DNA in nucleosomes and by the condensed structure adopted by chromatin formed on methylated DNA. Here, we discuss how oxidation of 5-methylcytosine into 5-hydroxymethylcytosine could provide the initial destabilizing clue for such transcription factors to get access to nucleosomal DNA and read epigenetic information.
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