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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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What is Gene Expression?01:42

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
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What is Gene Expression?01:36

What is Gene Expression?

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Chromatin Position Affects Gene Expression02:35

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Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
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mRNA Stability and Gene Expression02:51

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
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RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

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RNA Interference to Knock Down Gene Expression.

Haiyong Han1

  • 1Translational Genomics Research Institute, 445. N. Fifth St., Suite 400, Phoenix, AZ, 85004, USA. hhan@tgen.org.

Methods in Molecular Biology (Clifton, N.J.)
|February 10, 2018
PubMed
Summary
This summary is machine-generated.

This chapter details using synthetic short interfering RNA (siRNA) for gene silencing in mammalian cells. It covers transfection methods and potential challenges of RNA interference technology.

Keywords:
ElectroporationGene silencingRNA interferenceRNAiTransfectionsiRNA

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Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • RNA interference (RNAi) is a natural biological process for gene silencing.
  • Double-stranded RNA (dsRNA) targets specific messenger RNA (mRNA) for degradation.
  • RNAi is a valuable tool for studying gene function by reducing gene expression post-transcriptionally.

Purpose of the Study:

  • To provide detailed procedures for utilizing synthetic short interfering RNA (siRNA) to achieve gene silencing in mammalian cells.
  • To outline methods for delivering siRNA, including lipid-based transfection and electroporation.
  • To discuss potential challenges and problems encountered with siRNA technology.

Main Methods:

  • Gene silencing using sequence-specific, synthetic short interfering RNA (siRNA).
  • Delivery of siRNA via lipid-based transfection reagents.
  • Delivery of siRNA via electroporation techniques.

Main Results:

  • Successful induction of gene silencing in mammalian cells using synthetic siRNA.
  • Demonstration of effective siRNA delivery through both lipid-based transfection and electroporation.
  • Identification and discussion of potential challenges associated with siRNA technology.

Conclusions:

  • Synthetic siRNA is an effective tool for post-transcriptional gene silencing in mammalian cells.
  • Both lipid-based transfection and electroporation are viable methods for siRNA delivery.
  • Understanding potential challenges is crucial for successful application of siRNA technology in research.