Related Experiment Video
Updated: Feb 14, 2026
12:24
Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy
Published on: September 29, 2016
7.4K
Transmembrane Signal Transduction in Bacterial Chemosensing
1Department of Biology, Texas A&M University, 3258 TAMU, College Station, 77843, TX, USA. mike@bio.tamu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2018
Summary
This volume details methods for studying bacterial cell membrane communication and environmental responses. It covers techniques from whole-cell behavior to molecular signaling pathways for understanding chemotaxis.
Area of Science:
- Microbiology
- Cell Biology
- Biochemistry
Background:
- Bacteria communicate with their environment via the cell membrane.
- This communication is crucial for nutrient uptake, information transmission, and cellular responses.
- Understanding these processes is key to deciphering bacterial behavior.
Purpose of the Study:
- To present a comprehensive overview of methods for studying bacterial environmental sensing.
- To explore techniques for analyzing cell membrane-initiated signaling pathways.
- To provide insights into bacterial chemotaxis and chemosensory mechanisms.
Main Methods:
- Bacterial chemotaxis analysis at population and whole-cell levels.
- In vivo receptor function studies and cryo-electron microscopy (Cryo-EM) for chemoreceptor structure.
- Intracellular protein movement monitoring, high-throughput screening, and chemical tool development.
Main Results:
- The volume compiles state-of-the-art methodologies from leading experts.
- It covers a spectrum of techniques applicable to bacterial signal transduction.
- Focuses on understanding how bacteria sense and respond to their chemical environment.
Conclusions:
- This collection offers essential methods for investigating bacterial cell-surface signaling.
- It facilitates research into bacterial chemotaxis and environmental adaptation.
- Provides a valuable resource for researchers in microbiology and related fields.
Keywords:
Bacterial behaviorBioinformaticsChemical toolsChemoeffectorsChemoreceptorsMolecular dynamicsTracking cells and moleculesMore Related Videos
07:42Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
Published on: September 19, 2018
8.4K
08:51Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Published on: June 25, 2015
9.6K
Related Concept Videos
01:30Bacterial Signaling
41.4K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
41.4K
01:26Signal Transduction: Overview
11.8K
Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...
Typically, signal transduction involves three...
11.8K
01:16Transduction
1.9K
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
1.9K
01:24Intracellular Signaling Cascades
53.8K
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
53.8K
01:25Single-pass Transmembrane Proteins
6.8K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
6.8K
02:03What is Cell Signaling?
131.1K
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
131.1K