Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

65.5K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.5K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.9K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.9K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.4K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.4K
PCR01:32

PCR

238.6K
Overview
238.6K
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
Bioavailability Enhancement: Drug Solubility Enhancement01:16

Bioavailability Enhancement: Drug Solubility Enhancement

278
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
278

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

African Dacus (Diptera: Tephritidae: molecular data and host plant associations do not corroborate morphology based classifications.

Molecular phylogenetics and evolution·2009
Same author

Neuronal activity in dorsomedial frontal cortex and prefrontal cortex reflecting irrelevant stimulus dimensions.

Experimental brain research·2001
Same author

Effects of MK801 and neuroleptics on prepulse inhibition: re-examination in two strains of rats.

Pharmacology, biochemistry, and behavior·2001
Same author

Effects of administering cocaine at the same versus varying times of day on circadian activity patterns and sensitization in rats.

Behavioral neuroscience·2000
Same author

A link function approach to model heterogeneity of residual variances over time in lactation curve analyses.

Journal of dairy science·2000
Same author

Rule-dependent neuronal activity in the prefrontal cortex.

Experimental brain research·1999
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026
Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: Feb 14, 2026

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
06:19

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Published on: June 23, 2022

3.0K

Real-time multiplexed PCR using surface enhanced Raman spectroscopy in a thermoplastic chip.

S M Restaino1, I M White

  • 1Fischell Department of Bioengineering, University of Maryland, College Park, USA. ianwhite@umd.edu.

Lab on a Chip
|February 14, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a novel real-time polymerase chain reaction (PCR) integrated with surface-enhanced Raman spectroscopy (SERS) for rapid pathogen detection. The system enables simultaneous nucleic acid amplification and SERS detection in a single well, facilitating faster diagnostics.

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.3K
Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

3.5K

Related Experiment Videos

Last Updated: Feb 14, 2026

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
06:19

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations

Published on: June 23, 2022

3.0K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.3K
Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

3.5K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Molecular Diagnostics

Background:

  • Surface-enhanced Raman spectroscopy (SERS) offers sensitive detection for various analytes.
  • Current PCR-SERS assays require manual mixing, hindering real-time analysis.
  • Multiplexed SERS detection following PCR is desirable for diagnostics.

Purpose of the Study:

  • To develop a real-time PCR-SERS microsystem for simultaneous nucleic acid amplification and detection.
  • To enable real-time, quantitative PCR with multiplexed SERS readout.
  • To integrate PCR and SERS detection into a single, automated system.

Main Methods:

  • A laser-cut thermoplastic fluidic chip with a dialysis membrane was designed.
  • The chip separates the PCR reaction from a SERS-active silver colloid.
  • Degradation of a Raman-reporter-labeled DNA probe releases the reporter for SERS detection.

Main Results:

  • The microsystem achieved simultaneous nucleic acid amplification and real-time SERS detection.
  • The system enabled real-time, single-well multiplexed readout of PCR products.
  • Demonstrated simultaneous detection of two biomarker genes for MRSA.

Conclusions:

  • The developed real-time PCR-SERS microsystem integrates amplification and detection.
  • This approach eliminates manual steps and enables real-time monitoring.
  • The system shows promise for rapid and multiplexed pathogen detection, such as for MRSA.