Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Statistical Significance01:50

Statistical Significance

22.3K
Once data is collected from both the experimental and the control groups, a statistical analysis is conducted to find out if there are meaningful differences between the two groups. A statistical analysis determines how likely any difference found is due to chance (and thus not meaningful). In psychology, group differences are considered meaningful, or significant, if the odds that these differences occurred by chance alone are 5 percent or less. Stated another way, if we repeated this...
22.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.3K
Protein Complex Assembly02:41

Protein Complex Assembly

16.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K
Probability in Statistics01:14

Probability in Statistics

23.6K
Probability is the likelihood of an event occurring. The term event is defined as a collection of results of a procedure. An event is a simple event when an outcome cannot be divided into simpler parts.
An example of a simple event is a coin toss. The result of a coin toss is either a head or a tail. Here, head and tail are two simple events. These two simple events make up the sample space. Further, the probability of an event occurring falls within the range of 0 to 1. The probability of an...
23.6K
Introduction to Statistics01:17

Introduction to Statistics

65.0K
The science of statistics involves collecting, analyzing, interpreting, and presenting data. The method of collecting, organizing, and summarizing data is called descriptive statistics. The systematic method of drawing inferences from the sample data and predicting unknown characteristics of a population is called inferential statistics.
In statistics, the collection of individuals or objects under study is called population. The idea of sampling is to select a portion of the larger population...
65.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nuclear pore complex distribution on the nuclear envelope: Insights into curvature, chromatin, and actin contributions.

Physical review. E·2026
Same author

Better reporting is better science: Community-defined minimal reporting requirements for light microscopy.

The Journal of cell biology·2026
Same author

VAMP7-dependent late endosomal secretion of ER and mitochondrial proteins impacts the tumor microenvironment and macrophage engagement.

Nature communications·2026
Same author

Gut bioengineered models to study host-microbiota-probiotics interactions.

Microbiome research reports·2025
Same author

Morphometric study of bioprinted hydrogel deformation during histological preparation.

Journal of histotechnology·2025
Same author

Author Correction: Endophilin marks and controls a clathrin-independent endocytic pathway.

Nature·2025
Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026
Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026
Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026
Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026
Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026
Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Feb 14, 2026

Spatial Molecular Imaging of the Glycome Using Mass Spectrometry
08:52

Spatial Molecular Imaging of the Glycome Using Mass Spectrometry

Published on: November 28, 2025

600

Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics.

Thibault Lagache1,2, Alexandre Grassart3, Stéphane Dallongeville1

  • 1Institut Pasteur, BioImage Analysis Unit. CNRS UMR 3691. 25 rue du Docteur Roux, 75724, Paris Cedex 15, France.

Nature Communications
|February 17, 2018
PubMed
Summary
This summary is machine-generated.

We developed Statistical Object Distance Analysis (SODA), a novel method for precise molecular localization and spatial distribution analysis. SODA enhances standard co-localization techniques by considering cellular geometry and molecule densities for accurate molecular mapping.

More Related Videos

The Spatial Memory Game: Testing the Relationship Between Spatial Language, Object Knowledge, and Spatial Cognition
05:15

The Spatial Memory Game: Testing the Relationship Between Spatial Language, Object Knowledge, and Spatial Cognition

Published on: February 19, 2018

11.4K
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.8K

Related Experiment Videos

Last Updated: Feb 14, 2026

Spatial Molecular Imaging of the Glycome Using Mass Spectrometry
08:52

Spatial Molecular Imaging of the Glycome Using Mass Spectrometry

Published on: November 28, 2025

600
The Spatial Memory Game: Testing the Relationship Between Spatial Language, Object Knowledge, and Spatial Cognition
05:15

The Spatial Memory Game: Testing the Relationship Between Spatial Language, Object Knowledge, and Spatial Cognition

Published on: February 19, 2018

11.4K
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.8K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biophysics

Background:

  • Understanding protein function and molecular organization necessitates precise localization of molecules.
  • Standard co-localization techniques often lack the precision required for complex cellular structures.

Purpose of the Study:

  • To develop a novel statistical method, Statistical Object Distance Analysis (SODA), for precise molecular localization and spatial distribution analysis.
  • To improve upon standard co-localization techniques using micro- and nanoscopy.

Main Methods:

  • Development of the Statistical Object Distance Analysis (SODA) method.
  • Application of SODA with three-colour structured-illumination microscopy (SIM) on hippocampal neurons.
  • Utilisation of 3D-STORM for determining stoichiometry and distances between synaptic vesicle proteins.

Main Results:

  • SODA provides statistical maps of isolated and associated molecules, considering cellular geometry and densities.
  • Analysis of thousands of synapses revealed asymmetric triangular arrangement of presynaptic synapsin with postsynaptic markers homer and PSD95.
  • Homer was found to have a deeper localization compared to PSD95.

Conclusions:

  • SODA quantitatively assesses the geometry of molecular assemblies, offering insights into protein organization at the synapse.
  • The method significantly improves upon standard co-localization techniques for analyzing molecular spatial distributions.