Related Experiment Video
Updated: Feb 14, 2026

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
Published on: July 2, 2015
Immunoisolation of stem cells by simultaneous encapsulation and PEGylation
Roya Ramezanzadeh Andevari1, Sameereh Hashemi-Najafabadi2, Fatemeh Bagheri3
1Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Abstract:
Today, cell therapy is known as an important tool in the treatment of chronic diseases where cells lose their normal function. Immunoisolation systems using microencapsulation or PEGylation have been developed to evade the problem of rejection by the immune system. The aim of the present study was to investigate a combination of microencapsulation and PEGylation methods in coating mouse embryonic stem cells (mESCs) to determine its effect in reducing the host's immune response. Therefore, methoxy polyethylene glycol (mPEG) binding on alginate-trimethyl chitosan (TMC) microcapsules was investigated using FTIR. Furthermore, survival of the microencapsulated mESCs was confirmed using AO/PI staining and MTT assays. In addition, the effect of mESCs co-cultured with foreign lymphocytes was evaluated. Overall, interleukin-2 (IL-2) secretions as a response of the immune system revealed that mESCs microencapsulation in alginate-TMC-PEG, reduced the immune system response. The results suggested that IL-2 secretion was reduced to 62% at seventh day.
Related Concept Videos
Adult Stem Cells
Embryonic Stem Cells
Embryonic Stem Cells
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Somatic...
Distinctive Features of Adult Stem Cells vs Cancer Stem Cells
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...

