[ULTRASTRUCTURAL CHARACTERISTICS OF RATS TESTICLES UNDER SPERMATIC CORD STENOSIS AND AFTER BLOOD FLOW RESTORATION BY...

Hemodynamic disorders in the testicles cause chronic organ hypoxia with damage of its stroma and seminiferous tubules, which plays a leading role in the pathogenesis of the testicular form of male infertility development. The aim of the work was to establish the features of ultrastructural reorganization of the testicles tissue and its vascular bed under circulatory hypoxia conditions and after restoration of blood flow in the organ. The study was conducted on 84 white adult male rats. The control group consisted of 12 intact animals. The experimental group was divided into 3 series: with stenosis of the spermatic cord (48 animals), with stenosis of the spermatic cord and its recanalization on the 3rd day without correction (12 animals) and with stenosis of the spermatic cord and its recanalization with correction on the 3rd day (12 animals). Under conditions of dosage spermatic cord stenosis (when the ligature is applied) in the remote monitoring period, with electron microscopic study, were detected destructive changes of spermatid and spermatozoa, which were combined with significant focal intracellular and extracellular edema. In part of the cells heads were deformed, the acrosomes were sophisticated and fragmented. At one-stage decompression of the spermatic cord (removal of the stenosing ligature on the third day), the above-described changes in accordance were deepened. At 14th day of the observation, collagen fibers and an electron-transparent amorphous component were found in the perivascular space. When the proposed method of dosed decompression of the spermatic cord (successive removal of three stenosing ligatures of different diameters) has been applied changes in the testicles parenchyma and its intraorganic vessels were less severe. Functional activity of the testicle after correction of reperfusion changes, was not significantly affected, what was proved by the presence in seminiferous tubules lumen of spermatozoa mature forms.