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TRIM37 inhibits PDGF-BB-induced proliferation and migration of airway smooth muscle cells

Ying Dai1, Ying Li1, Ruiduo Cheng1

  • 1Department of Pediatrics, Henan University Huaihe Hospital, Kaifeng, 475000, Henan Province, PR China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|February 25, 2018
PubMed

Insights

Tripartite motif 37 (TRIM37) suppresses airway smooth muscle cell proliferation and migration. This protein inhibits the Wnt/β-catenin pathway, offering potential therapeutic targets for asthma.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Respiratory Medicine

Background:

  • Tripartite motif 37 (TRIM37) is a TRIM family protein implicated in asthma.
  • The role of TRIM37 in airway smooth muscle cell (ASMC) proliferation and migration remains unclear.

Purpose of the Study:

  • To investigate the effect of TRIM37 on platelet-derived growth factor BB (PDGF-BB)-stimulated ASMC proliferation and migration.
  • To explore the underlying molecular mechanisms of TRIM37 action.

Main Methods:

  • ASMCs were stimulated with PDGF-BB.
  • TRIM37 expression was manipulated (overexpressed).
  • Cell proliferation and migration assays were performed.
  • Western blotting analyzed protein expression (β-catenin, c-Myc, cyclinD1).
  • Wnt/β-catenin pathway activation was induced using LiCl.

Main Results:

  • PDGF-BB stimulation decreased endogenous TRIM37 expression in ASMCs.
  • TRIM37 overexpression inhibited PDGF-BB-induced ASMC proliferation and migration.
  • TRIM37 overexpression suppressed β-catenin, c-Myc, and cyclinD1 protein levels.
  • LiCl treatment reversed the inhibitory effects of TRIM37.

Conclusions:

  • TRIM37 inhibits ASMC proliferation and migration induced by PDGF-BB.
  • TRIM37 exerts its inhibitory effects by suppressing the Wnt/β-catenin signaling pathway.
  • TRIM37 represents a potential therapeutic target for asthma-related airway remodeling.
Keywords:
Airway smooth muscle cells (ASMCs)AsthmaMigrationProliferationTRIM37

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