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Plant reference genes for development and stress response studies.

Joyous T Joseph1, Najya Jabeen Poolakkalody, Jasmine M Shah

  • 1Department of Plant Science, Central University of Kerala, Padannakkad, Kasaragod 671 314, India.

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|February 28, 2018
PubMed
Summary
This summary is machine-generated.

Selecting stable reference genes for gene expression analysis is crucial but challenging, as their expression varies with experimental conditions. This review guides researchers in choosing appropriate reference genes for 16 plant species across various conditions.

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Area of Science:

  • Plant Molecular Biology
  • Gene Expression Analysis
  • Bioinformatics

Background:

  • Reference genes are vital for normalizing gene expression data in RNA/DNA experiments.
  • Expression stability of reference genes is highly dependent on experimental conditions, necessitating careful selection.
  • Previous studies have explored numerous genes for normalization, but a consolidated guide is lacking.

Purpose of the Study:

  • To review and consolidate information on reference genes used in gene expression analyses across diverse plant species.
  • To categorize reference genes based on plant species and experimental conditions (biotic/abiotic stress, development, tissues).
  • To provide a comprehensive guide for selecting suitable reference genes for accurate gene expression studies.

Main Methods:

  • Literature review of published studies on gene expression analysis in plants.
  • Compilation of approximately 120 genes used as reference genes.
  • Categorization of genes by plant species (16 species included) and experimental conditions.

Main Results:

  • Identified approximately 120 genes utilized for normalization in gene expression studies.
  • Detailed plant-wise categorization of reference genes used under biotic stress, abiotic stress, developmental stages, and various organs/tissues.
  • Included a wide range of plant species, encompassing both model plants and major crop species from monocot and dicot classes.

Conclusions:

  • Reference gene selection is condition-dependent and critical for reliable gene expression data.
  • This review offers a valuable resource for researchers to identify and select appropriate reference genes for their specific plant systems and experimental contexts.
  • The consolidated information aids in improving the accuracy and reproducibility of gene expression studies in plants.