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Antimicrobial Efficacy of a New Chlorhexidine-based Device Against Staphylococcus aureus Colonization of Venous

Paulina M Kowalewska1, Shawn M Petrik, Attilio E Di Fiore

  • 1Thrombosis and Atherosclerosis Research Institute and Department of Medicine, McMaster University, Hamilton, Ontario, Canada (Dr Fox-Robichaud); Department of Medical Biophysics, University of Western Ontario, London, Ontario, Canada (Dr Kowalewska); Comparative Medicine, Thrombosis and Atherosclerosis Research Institute, McMaster University, Hamilton, Ontario, Canada (Dr Petrik); and ATTWILL Medical Solutions, Inc, West Jordan, Utah (Mr Di Fiore). Paulina M. Kowalewska, PhD, was an Ontario Centres of Excellence TalentEdge Postdoctoral Fellow at McMaster University during this study and is currently a postdoctoral associate at the University of Western Ontario. Shawn M. Petrik, DVM, MSc, Dip LAM, is the leader of the Comparative Medicine Group at McMaster University, which supports veterinarians, physicians, and scientists who study animal models of human diseases. Attilio E. Di Fiore, BSc, BScEng, PEng, is a biomedical engineer specializing in medical device design and development, with extensive experience in vascular device manufacturing and infection control. Alison E. Fox-Robichaud, MD, MSc, FRCPC, is a clinician scientist and professor of medicine with research interests in inflammation and sepsis. She is also a collaborator on various multicenter knowledge translation studies through the Canadian Critical Care Translational Biology Group and the Canadian Critical Care Trials Group.

Journal of Infusion Nursing : the Official Publication of the Infusion Nurses Society
|March 1, 2018
PubMed

Insights

ChloraLock, an antimicrobial device, significantly reduces bacterial contamination in vascular catheters. This study demonstrates its efficacy in vitro and in vivo, crucial for preventing bloodstream infections.

Area of Science:

  • Infectious Diseases
  • Medical Devices
  • Antimicrobial Research

Background:

  • Vascular catheters are a primary source of nosocomial bloodstream infections.
  • Antimicrobial lock solutions are essential for preventing catheter-related infections.

Purpose of the Study:

  • To evaluate the antimicrobial efficacy of ChloraLock, a novel chlorhexidine digluconate (CHG) device.
  • To assess ChloraLock's ability to reduce Staphylococcus aureus catheter contamination in a swine model.

Main Methods:

  • In vitro antimicrobial testing of ChloraLock.
  • In vivo study using Yorkshire swine with external jugular vein catheters.
  • Quantification of bacterial load reduction using colony-forming units (CFU).

Main Results:

  • ChloraLock demonstrated significant bacterial load reduction up to 6 log10 CFU in vitro.
  • In vivo, ChloraLock reduced Staphylococcus aureus contamination by 3 to 4 log10 CFU/lumen.
  • The device effectively infused CHG into the catheter lumen during locking with 0.9% NaCl.

Conclusions:

  • ChloraLock exhibits potent antimicrobial efficacy against Staphylococcus aureus.
  • The device is effective in reducing catheter contamination in both laboratory and animal models.
  • ChloraLock presents a promising strategy for preventing vascular catheter-associated infections.

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