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Mid-infrared multispectral tissue imaging using a chalcogenide fiber supercontinuum source.

Christian Rosenberg Petersen, Nikola Prtljaga, Mark Farries

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    This study demonstrates mid-infrared supercontinuum (SC) tissue imaging beyond 5 µm. A novel fiber-coupled SC source enabled high-resolution imaging of colon tissue, advancing optical diagnostic capabilities.

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    Area of Science:

    • Biomedical Optics
    • Materials Science
    • Infrared Spectroscopy

    Background:

    • Mid-infrared (MIR) imaging offers unique molecular contrast for tissue analysis.
    • Existing MIR imaging techniques face limitations in bandwidth, power, or resolution.
    • Supercontinuum (SC) sources provide broad spectral coverage for advanced imaging.

    Purpose of the Study:

    • To demonstrate mid-infrared supercontinuum (SC) tissue imaging beyond 5 µm.
    • To showcase a novel fiber-coupled SC source for enhanced imaging performance.
    • To evaluate the potential of this technology for biomedical applications.

    Main Methods:

    • Generation of a broad bandwidth (2-7.5 µm) SC in a tapered chalcogenide photonic crystal fiber.
    • Utilizing a fiber-coupled SC source for diffraction-limited imaging.
    • Point scanning transmission imaging of colon tissue at selected MIR wavelengths (5.7-7.3 µm).

    Main Results:

    • Successful demonstration of SC tissue imaging at wavelengths > 5 µm.
    • Achieved broad spectral coverage and high average power from the SC source.
    • Obtained high-resolution images of colon tissue, comparable to commercial instruments.

    Conclusions:

    • Mid-infrared SC tissue imaging beyond 5 µm is feasible with advanced fiber-based sources.
    • This technology offers a promising tool for label-free molecular imaging in biomedical research.
    • The developed SC source enables high-performance, diffraction-limited imaging for tissue diagnostics.