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Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
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In Vivo Study of Mastoid Obliteration Using Hydroxyapatite-Chitosan Patch
Journal of Biomedical Nanotechnology
|March 2, 2018
Summary
A new hydroxyapatite-chitosan patch offers superior mastoid cavity obliteration compared to traditional materials. This flexible, biocompatible patch demonstrates excellent osteoconductivity and handling for otologic surgery.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Otolaryngology
Background:
- Artificial materials are used for mastoid cavity obliteration after canal wall down tympanomastoidectomy.
- Hydroxyapatite is a popular material but suffers from brittleness.
- Combining hydroxyapatite with chitosan creates a flexible, biocompatible composite patch.
Purpose of the Study:
- To evaluate the efficacy of a novel hydroxyapatite-chitosan patch for tympanic cavity obliteration in a rat model.
- To compare the patch's performance against sham surgery, bone cement, and homologous cartilage.
Main Methods:
- A hydroxyapatite-chitosan patch was fabricated using sublimation-assisted compression.
- The patch was implanted in a rat model for tympanic cavity obliteration.
- Evaluation involved computed tomography, histology, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy.
Main Results:
- The hydroxyapatite-chitosan patch group showed a significantly superior tympanic cavity obliteration ratio (40.96%) compared to bone cement (16.07%) and homologous cartilage (32.23%).
- New bone formation ratios were comparable across all tested groups, ranging from 27.92% to 29.17%.
- The patch exhibited easy handling, flexibility, and favorable osteogenesis and biostability.
Conclusions:
- The hydroxyapatite-chitosan patch is a promising material for mastoid cavity obliteration.
- Its mechanical properties and biocompatibility make it an effective alternative to existing materials.
- Further research may support its clinical application in otologic procedures.
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